no interuptions.... yes
quickly, within reason. Dont hurry as the silicone will be going off slowly but you also dont want to muck arround
Most tanks are built from the base up. bback goes on base, both sides next then the front.
Put a strip of masking tape around the ends to holh it all together.
Make sure that you feel the base on the outsides to make sure that the sides are lining up (dont just rely on looking as the silicone will be a bit deciving)
Silicon takes 1 day per mm of thicknes to cure so if you put the tank together today, dont think about filling it for a week. This also allows the fumes to come out.
After 24 hours, look through the glass at your joins for air bubbles and see if the air bubbles all join up which will allow a possible leak, these may need touching up.
Dont put the bits together then pull apart and back together as this will create air bubbles and therefore leaks
Make sure that you clean the glass as much as possible before reconstructing as cleaning now is easier than later