Here is a quote from "Jacstat" at RC, he is a reefer, and by trade a scientist of molecular immunology.
I post it as it can put to rest the idea that there is no point in dosing bacteria to an already cycled tank. -
"As far as biology, I don't know much about Zeo. What I do know is that if one wants to introduce a new complement of bacteria to a closed environment, one would not want to do it quickly. A small inoculation would be adequate, especially considering that the inoculation is repeated every day or two, over weeks. It would be strange to me if Zeobak did contain a high concentration of bacteria. As I've said earlier, these are living organisms that need to adjust and grow into their new environment. You can't bring these populations up and into equilibrium overnight. This is basic biology.
As for viability, I see no reason why chemolithotrophs can't be kept in a bottle at room temp for months, particularly at the concentrations described (10e6/mL).
As for contamination, I think that's ridiculous. If you think that it's any kind of feat to isolate and expand cultures without contamination then I'm not sure if whose intelligence you're insulting. This was doable 100 years ago. Solbby and I do this sort of things everyday, sometimes with irreplaceable samples."