Probally not, if the tank your going to put it in is already cycled, then the ammonia levels will be 0 so the bacteria will have nothing to eat. Just do a fish less cycle. I never use any products When cycling my tank, just never helps, with the exception of stress zyme to remove the chlorine and chloromines. It could work, I've seen some stuff on YouTube, he got a sponge(from a cycled tank) a cup and squeezed the contents of the sponge into the cup, the gunky stuff. I would like to verify if this works?
Good luck