livingart Posted June 8, 2010 Report Share Posted June 8, 2010 we are going to have an attenpt at geting some phyto plankton and rotifers cultured the ultimate goal is to try and raise some marine clown fish cricketman is the techy in charge of this that way if it is a failure i can blame him equipment list 90cm flourescent light 2 x 90cm, 40 Watt fluorescent bulbs On/off Timer Air pump 3 metre. Flexible airline tubing 1 metre. Rigid airline tubing Multi-outlet airline splitter/valve ■6 x 1 litre drink bottles Aquarium salt f/2 Formula Phytoplankton starter culture, Nannochloropsis occulata and a cabinet to keep it warm since winter is here Quote Link to comment Share on other sites More sharing options...
Heir Posted June 8, 2010 Report Share Posted June 8, 2010 Fun. Good luck :bounce: Quote Link to comment Share on other sites More sharing options...
ryanjury Posted June 8, 2010 Report Share Posted June 8, 2010 Sounds like a good project good luck.. I know there was someone who used to breed clowns in NZ have you got in touch with them for advice I believe they were forum members as well.. Quote Link to comment Share on other sites More sharing options...
livingart Posted June 8, 2010 Author Report Share Posted June 8, 2010 been there done that i am hoping it is not harder than raising seahorses been doing my research start of cabinet to keep it in something to hold the phyto bottles the bottles, to be sterilised yet cricketman drank them all stand for BBS syringes and airline filters Quote Link to comment Share on other sites More sharing options...
Cricketman Posted June 8, 2010 Report Share Posted June 8, 2010 We got nannochloropsis( green phyto), Pavlova lutheri (brown phyto), and a third "tetra" (haven't found the proper latin name yet) Experiment is to look into the different growth rates and survival rates of the fry using these 3 algae to feed directly, and gut-load artemia/ daphnia with. various algae have different nutritional values, between protein, lipids etc. and you can't blame me Mr "F/2 in my fridge" Quote Link to comment Share on other sites More sharing options...
livingart Posted June 9, 2010 Author Report Share Posted June 9, 2010 tops for phyto bottles frits on intakes filter down to 2 microns, syringes on exhaust with filter wool to preclude any backdraft whole setup, everything sterilised so far need to mix sea salt and RO/DI water then sterilise that too will adjust lighting Quote Link to comment Share on other sites More sharing options...
SamH Posted June 9, 2010 Report Share Posted June 9, 2010 "No officer, I'm not cooking P. Honest!" :lol: Quote Link to comment Share on other sites More sharing options...
livingart Posted June 9, 2010 Author Report Share Posted June 9, 2010 "P" is for phyto plankton Quote Link to comment Share on other sites More sharing options...
SamH Posted June 9, 2010 Report Share Posted June 9, 2010 Touche Quote Link to comment Share on other sites More sharing options...
phoenix44 Posted June 9, 2010 Report Share Posted June 9, 2010 looking perfect! Quote Link to comment Share on other sites More sharing options...
livingart Posted June 9, 2010 Author Report Share Posted June 9, 2010 thanks, cricketman spent awhile today drilling tops and marrying up tubing to fit tops properly going to put "Ts" on airline for BBS to allow removal of BBs through base Quote Link to comment Share on other sites More sharing options...
livingart Posted June 10, 2010 Author Report Share Posted June 10, 2010 we spent awhile mixing salt and RODI water today then had to sterilse the mix so now bottles are filled with salt water to the correct salinity F2 media inserted wait for it to cool down a bit then to add phyto trialling a waterbed heat pad for keeping it all at the right temp Quote Link to comment Share on other sites More sharing options...
Cricketman Posted June 10, 2010 Report Share Posted June 10, 2010 Made the decision to keep rotifers well clear of this phyto setup for now, and do them in a seperate area completely, with bleach baths between handling to ensure no cross-contamination. Doing that alreay for the phyto. Got two "working cultures" (ones to feed out) going, one Pavlova lutheri and one Nannochloropsis sp. Next step for me is to develop a "clean" cupboard (laminar cabinet) to store "parent cultures". These will act as redundant cultures just to make sure that we Don't lose a species of phyto, and can keep innoculating "working cultures". As such, these cultures are kept a little cooler, and as Aseptic as possble. This should be achieved with wipeable, non-porous material , with a positive internal pressure, and hopefully a "air dam"/"air curtain" accross the front acting when the cabinet is open. This coupled with ethanol to disinfect everything in the cabinet and equipment (ethanol does not leave a residue when it evaporates) should give us a good chance of success in keeping cultures alive for a long time (hopefully perpetually). Currently waiting on a third species of phytoplankton from toppom, and then waiting for Mr. & Mrs. Amphiprion ocellaris to practise saving the species... :lol: which, judging by thier behaviour, should be soon. Cue: bow chika wow wow! :lol: Quote Link to comment Share on other sites More sharing options...
livingart Posted June 27, 2010 Author Report Share Posted June 27, 2010 well we have geared the factory up just added this courtesy of toppom tetra phyto and rotifers now rearing mysis/mysid and brine shrimp Quote Link to comment Share on other sites More sharing options...
Nymox Posted June 27, 2010 Report Share Posted June 27, 2010 looking good guys! not to far off raising some clown fry then Quote Link to comment Share on other sites More sharing options...
livingart Posted June 27, 2010 Author Report Share Posted June 27, 2010 looking good guys! not to far off raising some clown fry then day six since they were laid cleaner shrimp is carrying as well Quote Link to comment Share on other sites More sharing options...
Nymox Posted June 27, 2010 Report Share Posted June 27, 2010 oh cool! I bet clown fry are cute. Quote Link to comment Share on other sites More sharing options...
livingart Posted July 4, 2010 Author Report Share Posted July 4, 2010 jars are pumping brine shrimp are breeding, the big ones with the black circles on them are females with eggs not a long vid though and 3rd generation mysid shrimp Quote Link to comment Share on other sites More sharing options...
Insect Direct Posted July 4, 2010 Report Share Posted July 4, 2010 awesome those brine shrimp must be dizzy Quote Link to comment Share on other sites More sharing options...
livingart Posted July 4, 2010 Author Report Share Posted July 4, 2010 felt sorry for them and turned the air down they still went for a spin Quote Link to comment Share on other sites More sharing options...
Insect Direct Posted July 4, 2010 Report Share Posted July 4, 2010 :lol: they must be happy if theyre breeding. Quote Link to comment Share on other sites More sharing options...
livingart Posted July 5, 2010 Author Report Share Posted July 5, 2010 now we can see what we are producing Quote Link to comment Share on other sites More sharing options...
cookie extreme Posted July 11, 2010 Report Share Posted July 11, 2010 been a while! any updates? how are the baby clowns doing? Quote Link to comment Share on other sites More sharing options...
livingart Posted July 11, 2010 Author Report Share Posted July 11, 2010 maroons haven't hatched yet ocellaris missed the first lot to go free swimming 2nd lot are 2 days free swimming will post pics when bigger and can focus on them Quote Link to comment Share on other sites More sharing options...
cookie extreme Posted July 16, 2010 Report Share Posted July 16, 2010 second lot must be doing fine by now! any pics? Quote Link to comment Share on other sites More sharing options...
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